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86
Charles River Laboratories c57bl 6 j wildtype mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
C57bl 6 J Wildtype Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c57bl 6 j wildtype mice/product/Charles River Laboratories
Average 86 stars, based on 1 article reviews
c57bl 6 j wildtype mice - by Bioz Stars, 2026-06
86/100 stars
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86
Charles River Laboratories c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
C57bl 6 J Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c57bl 6 j mice/product/Charles River Laboratories
Average 86 stars, based on 1 article reviews
c57bl 6 j mice - by Bioz Stars, 2026-06
86/100 stars
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86
Jackson Laboratory c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
C57bl 6 J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c57bl 6 j mice/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
c57bl 6 j mice - by Bioz Stars, 2026-06
86/100 stars
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86
Jackson Laboratory adult c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Adult C57bl 6 J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adult c57bl 6 j mice/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
adult c57bl 6 j mice - by Bioz Stars, 2026-06
86/100 stars
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86
Oriental Yeast Co male c57bl 6 j mice aged 9
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Male C57bl 6 J Mice Aged 9, supplied by Oriental Yeast Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/male c57bl 6 j mice aged 9/product/Oriental Yeast Co
Average 86 stars, based on 1 article reviews
male c57bl 6 j mice aged 9 - by Bioz Stars, 2026-06
86/100 stars
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86
Jackson Laboratory adult male c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Adult Male C57bl 6 J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adult male c57bl 6 j mice/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
adult male c57bl 6 j mice - by Bioz Stars, 2026-06
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86
Changsheng Bio Technology Co male c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Male C57bl 6 J Mice, supplied by Changsheng Bio Technology Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/male c57bl 6 j mice/product/Changsheng Bio Technology Co
Average 86 stars, based on 1 article reviews
male c57bl 6 j mice - by Bioz Stars, 2026-06
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86
Jackson Laboratory animals wild type c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Animals Wild Type C57bl 6 J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/animals wild type c57bl 6 j mice/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
animals wild type c57bl 6 j mice - by Bioz Stars, 2026-06
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86
Charles River Laboratories organoid derived 2d monolayers c57bl 6 j mice
From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized <t>C57BL/6</t> J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.
Organoid Derived 2d Monolayers C57bl 6 J Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/organoid derived 2d monolayers c57bl 6 j mice/product/Charles River Laboratories
Average 86 stars, based on 1 article reviews
organoid derived 2d monolayers c57bl 6 j mice - by Bioz Stars, 2026-06
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Image Search Results


From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized C57BL/6 J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.

Journal: STAR Protocols

Article Title: Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103 + cDC1s and CD301b + cDC2s

doi: 10.1016/j.xpro.2026.104508

Figure Lengend Snippet: From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized C57BL/6 J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.

Article Snippet: C57BL/6 J wildtype mice (female, between 8-15 weeks of age) , Charles River , #C57/BL6′′J′′.

Techniques: Single Cell, Suspension, Isolation, Gradient Centrifugation

Identification of myeloid progenitors in murine bone marrow (A) Annotation of myeloid progenitors in bone marrow single cell suspensions of C57BL/6 J mice via flow cytometry. The provided annotation approach is based on the combined work of Liu et al. and Schlitzer et al. (B) Dimensionality reduction via Uniform Manifold Approximation and Projection (UMAP) of 30,000 cells from CD45+DAPI-LIN- cells. Gates in (A) were mapped onto the UMAP and color coded (left) while visualizing the expression of population defining markers as expression heat maps (right). Unbiased analysis was performed using the FlowJo plugins downsample and UMAP. LIN=lineage; UMAP=uniform manifold approximation and projection; CMP=common myeloid progenitor; GMP=granulocyte-macrophage progenitor; GP=granulocyte progenitor; cMoP=common monocyte progenitor; M.=monocytes; MDP=monocyte-dendritic cell progenitor; CDP=common dendritic cell progenitor.

Journal: STAR Protocols

Article Title: Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103 + cDC1s and CD301b + cDC2s

doi: 10.1016/j.xpro.2026.104508

Figure Lengend Snippet: Identification of myeloid progenitors in murine bone marrow (A) Annotation of myeloid progenitors in bone marrow single cell suspensions of C57BL/6 J mice via flow cytometry. The provided annotation approach is based on the combined work of Liu et al. and Schlitzer et al. (B) Dimensionality reduction via Uniform Manifold Approximation and Projection (UMAP) of 30,000 cells from CD45+DAPI-LIN- cells. Gates in (A) were mapped onto the UMAP and color coded (left) while visualizing the expression of population defining markers as expression heat maps (right). Unbiased analysis was performed using the FlowJo plugins downsample and UMAP. LIN=lineage; UMAP=uniform manifold approximation and projection; CMP=common myeloid progenitor; GMP=granulocyte-macrophage progenitor; GP=granulocyte progenitor; cMoP=common monocyte progenitor; M.=monocytes; MDP=monocyte-dendritic cell progenitor; CDP=common dendritic cell progenitor.

Article Snippet: C57BL/6 J wildtype mice (female, between 8-15 weeks of age) , Charles River , #C57/BL6′′J′′.

Techniques: Single Cell, Flow Cytometry, Expressing